Leja counting chamber (Neubauer)
The Leja counting chamber is characterized by the distance between the slide and the cover glass which is exactly 100 µm. (Thickness of the cover glass: 0,44 mm)
|Product description||Product code||Unit|
|Leja counting chamber 100 micron 2 chambers||SC 100-01-02-A||1 unit = 25 piece|
|counting grid in ocular||SC 100-01-02-B||1 unit = 25 piece|
Product facts and notices
The counting grid is built by 3 x 3 so called large squares of an expanse of 1 mm2 reach. For the counting of spermatozoa the center large square is to be used. This square is built by 5 x 5 group squares. Each group square has an expanse of 0,04 mm2 and a volume of 4 µm3.
The group squares are enframed by three lines each. The middle one of these lines is the demarcation of the group squares. The group squares are subdivided into 4 x 4 mini squares with an expanse of 0,0025 mm2 each.
- When counting 250 sperms in 5 group squares, that matches 250 sperms in a total volume of 5 x 4 µm = 20 µm3.
- According to that you have 250 sperms in 1 µm3/ 20 µm3 = 12,5 Sperms.
Conversion to Mio./ml:
= 12.500 sperms/mm3
= 12.500.000 sperms/cm3
= 12,5 x 106 sperms/ml
= 12,5 Mio. sperms/ml
SPgez. x Verd.
AGQ x TK x GPgez
SPgez. x Verd.
VGQ x GPgez.
Conversion to Mio./ml using factor 1000
|SPgez.||=||total sperms counted|
|Verd.||=||factor of dilution|
|AGQ||=||expanse of a group square in mm2|
|TK||=||depth of the chamber in mm|
|GPgez.||=||number of counted group squares|
|VGQ||=||volume of a group square|