Leja counting chamber (Neubauer)
The Leja counting chamber is characterized by the distance between the slide and the cover glass which is exactly 100 µm. (Thickness of the cover glass: 0,44 mm)
| Product description | Product code | Unit |
|---|---|---|
| Leja counting chamber 100 micron 2 chambers | SC 100-01-02-A | 1 unit = 25 piece |
| counting grid in ocular | SC 100-01-02-B | 1 unit = 25 piece |
Product facts and notices
The counting grid is built by 3 x 3 so called large squares of an expanse of 1 mm2 reach. For the counting of spermatozoa the center large square is to be used. This square is built by 5 x 5 group squares. Each group square has an expanse of 0,04 mm2 and a volume of 4 µm3.
The group squares are enframed by three lines each. The middle one of these lines is the demarcation of the group squares. The group squares are subdivided into 4 x 4 mini squares with an expanse of 0,0025 mm2 each.
Spermcounting example
- When counting 250 sperms in 5 group squares, that matches 250 sperms in a total volume of 5 x 4 µm = 20 µm3.
- According to that you have 250 sperms in 1 µm3/ 20 µm3 = 12,5 Sperms.
Conversion to Mio./ml:
12,5 sperms/µm3
= 12.500 sperms/mm3
= 12.500.000 sperms/cm3
= 12,5 x 106 sperms/ml
= 12,5 Mio. sperms/ml
Calculation formula:
SPgez. x Verd.
AGQ x TK x GPgez
=
SPgez. x Verd.
VGQ x GPgez.
= Spermien/µl
Conversion to Mio./ml using factor 1000
| SPgez. | = | total sperms counted |
| Verd. | = | factor of dilution |
| AGQ | = | expanse of a group square in mm2 |
| TK | = | depth of the chamber in mm |
| GPgez. | = | number of counted group squares |
| VGQ | = | volume of a group square |