SpermFilterⓇ

SpermFilter® is a density gradients used in ART for separation and purification of highly motile human spermatozoa.

Product description Product code Unit
SpermFilter 100% 3SF0050 50 ml
SpermFilter 100% 3SF0100 100 ml
SpermFilter 100% 3SF0250 250ml
SpermFilter 80% 3SFG80-0010 10 ml
SpermFilter 80% 3SFG80-0050 50 ml
SpermFilter 80% 3SFG80-0100 100 ml
SpermFilter 80% 3SFG80-0250 250 ml
SpermFilter 45% 3SFG40-0010 10 ml
SpermFilter 45% 3SFG40-0050 50 ml
SpermFilter 45% 3SFG40-0100 100 ml
SpermFilter 45% 3SFG40-0250 250 ml

Product facts and notices

SpermFilter 100%

  • Is composed of silica particles covalently coated with silane and resuspended in isotonic phosphate and Hepes buffered saline.
  • Water, CaCi2.2H2O, KCl, MgSO4.7H2O, NaCl, NaH2PO4.2H2O, glucose, NaHCO3, Na-pyruvate, HEPES, and silanised silica.

SpermFilter 40/80%

  • Water, CaCl2.2H2O, KCl, MgSO4.7H2O, NaCl, NaH2PO4.2H2O, glucose, NaHCO3, Na-pyruvate, HEPES, phenol red, gentamicin, human serum albumin and silanised silica.
  • The medium is sterilized by filtration and sterility is ensured by testing each batch. Sterility tests verify the absence of viable micro-organism (bacteria, yeast and fungi).
  • SpermFilter is manufactured under sterile conditions. Sterility tests for each batch verify the absence of viable microorganisms. The limit of endotoxines is < 0,25 EU/ml. The manufacturer Gynotec B.V., Netherlands, produces according to a quality managements system under ISO 13485:2003 & EN ISO 13485:2012.
  • The shelf life from SpermFilter 100% is two years from production date when stored refigerated (2°C – 8°C).
  • The shelf life from SpermFilter 80% and 45% is 18 months from production date when stored refigerated (2°C – 8°C).
pH 7,40 ± 0,2
Osmolality (mOsm/kg) SpermFilter 100%

300 ± 10

SpermFilter 40/80

290 ± 10

Sterility sterile – SAL 10-3 (Sterility Assurance Level)
Endotoxins (ng/ml) < 0,1
Sperm Motility Test

 

 

 

  • Prior to usage, dilute SpermFilter® (100 %) with Hepes buffered isotonic medium (e.g. SpermWash®) at room temperature (e.g. 8 Vol SpermFilter® and 2 Vol SpermWash®).
  • Pipet 2 ml 40 % SpermFilter® into a centrifuge tube. Gently underlay 2 ml SpermFilter® gradient 80%.
  • Carefully layer the liquified semen on top of the gradient and centrifuge at 300 x g for 20 min (in case that after 20 min no pellet is visible, centrifuge for another 10 min).
    • Aspirate the supernatant and resuspend the pellet with fresh SpermWash®. Centrifuge for 10 more min. Repeat this step once to achieve a maximum effect of cleansing.
  • Finally aspirate the supernatant to have the pellet resuspended for the following procedures of reproductive medicine.