GM501 SpermMobil
GM501 SpermMobil is a HEPES-buffered reagent containing low bicarbonate. It is used for in-vitro examination of sperms of necrozoospermic ejaculates as well as of immotile sperms isolated from testicular tissue (TESE).
Rev03_00
| Product description | Product code | Unit |
|---|---|---|
| GM501 SpermMobil | 4 GM 501SMOBIL-5 | 5 ml |
| GM501 SpermMobil | 4 GM 501SMOBIL-5-S | 1 ml |
Product facts and notices
- Composition
- Product specifications and quality control
- Instructions for use
- Precautions and warnings
- Storage instructions and stability
Composition:
- NaCl, KCl, KH2PO4, MgSO4.7H2O, NaHCO3, CaCl2.2H2O, D(+)-Glucose Anhydrous, Na-Lactate, Na-Pyruvate, EDTA, Alanyl-Glutamine, water, non-essential and essential Amino Acids, HEPES, Theophylline, Phenol Red.
Product specifications and quality control:
- All raw materials are of highest available purity (European Pharmacopoeia and/or USP standard) if applicable.
- A certificate of analysis is available for each batch upon request from our website with respective lot number.
- The MSDS for GM501 SpermMobil is available upon request and can be downloaded from our website.
- GM501 SpermMobil is manufactured and tested according to the following specifications:
| pH at 37°C) | 7,20-7,60 |
| Osmolality (mOsm/kg) | 280-320 |
| Sterility | sterile – SAL 10-3 (Sterility Assurance Level) |
| Endotoxins (EU/ml) | < 0,50 |
|
Sperm Survival test (%) survival after 45 minutes exposure of density selected spermatozoa to the test medium |
≥ 80% |
Instructions for use:
- The intended users are IVF professionals (biologists, lab technicians, embryologists or medical doctors).
- Do not equilibrate GM501 SpermMobil in a CO2-incubator, just warm it up to 37°C. GM501 SpermMobil is HEPES-buffered. Incubation in a CO2-incubator will lower the pH.
- To facilitate sperm-activation add 3-4 μl GM501 SpermMobil to the sperm-containing drop (approx. 30-40μl/ditution 1:10) in sperm preparation-medium inside the Petri-dishes.
- GM501 SpermMobil should be added to the opposing side from where the sperms are to be aspirated.
- Wait for 10 minutes. The activating effect initiates after a few minutes and lasts for up to one hour.
Precautions and warnings:
- Handle all specimens as if capable of transmitting HIV or hepatitis. Always wear protective clothing when handling specimens.
- Always work under strict hygienic conditions (ISO 5 environment, e.g. LAF-bench) to avoid possible contamination.
- Only for the intended use.
Pre-use checks:
- Do not use the product if bottle, seal of the container or package is opened or defective when the product is delivered.
- The concentration of the xanthine derivative Theophylline leads to the occasional formation of precipitate at 2-8°C storage temperature. It is dissolved completely when warmed at 37°C (within 24 h) and does not compromise function and safety of the product.
- Do not use the product if it becomes discoloured, cloudy or shows any evidence of microbial contamination.
Storage instructions and stability:
- Shelf life is 6 months from time of manufacture.
- Store between 2-8°C.
- Do not freeze before use.
- Keep away from (sun)light.
- The product can be used safely up to 7 days after opening, when sterile conditions are maintained and the products are stored at 2-8°C.
- Do not use after expiry date.